wild type meg 01 cells  (ATCC)

Journal: PLOS Pathogens

Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles

doi: 10.1371/journal.ppat.1013985

Figure Lengend Snippet: A. Percentage of GFP-positive MEG-01 VP30 cells. PMA-treated or untreated cells were exposed to EBOVΔVP30-GFP at an MOI of 5 and GFP-positive cells were quantified by flow cytometry 2 days after exposure. Data are representative of two independent experiments. B. EBOVΔVP30 titers from MEG-01 VP30 cells. PMA-treated or untreated cells were exposed to EBOVΔVP30-GFP at an MOI of 1. Supernatants were collected daily, and viral titers were determined using Vero VP30 cells. Data are presented as means ± SD of three independent experiments. The dotted lines indicate the lower limit of detection (20 ffu/ml). Statistical significance was assessed by using the multiple unpaired t-test. * p < 0.05, ** p < 0.01.

Article Snippet: Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics.

Techniques: Flow Cytometry

Journal: PLOS Pathogens

Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles

doi: 10.1371/journal.ppat.1013985

Figure Lengend Snippet: A. Detection of EBOV proteins by western blot from PLPs released form EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were collected on day 4 post-exposure from PMA-treated MEG-01 WT or VP30 cells exposed to EBOVΔVP30 at an MOI of 5. The indicated proteins were analyzed by immunoblotting. Data are representative of two independent experiments. B. Relative amount of EBOV gRNA in PLPs released from EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were prepared as described in (A). EBOV gRNA was quantified by RT-qPCR using the indicated genome-specific primer pairs and normalized to the gRNA in PLPs from MEG-01 WT cells. Data are presented as means ± SD from two independent experiments performed in triplicate. C. Localization of EBOV GP in PLPs released from EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were prepared as described in (A). The platelet marker CD41 (magenta) and EBOV GP (green) were visualized by immunofluorescence microscopy using specific antibodies, along with the corresponding bright-field image. Scale bars, 5 μm. D. Interaction between EBOV NP/VP35 (top panel) and NP/VP40 (bottom panel) in PLPs released from EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were prepared as described in (A). NP/VP35 and NP/VP40 complexes (green) were visualized by using a proximity ligation assay (PLA) with specific antibodies and overlaid on the corresponding bright-field image. Scale bars, 5 μm.

Article Snippet: Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics.

Techniques: Western Blot, Quantitative RT-PCR, Marker, Immunofluorescence, Microscopy, Proximity Ligation Assay

Journal: PLOS Pathogens

Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles

doi: 10.1371/journal.ppat.1013985

Figure Lengend Snippet: Relative expression levels of EBOV mRNA (A) and gRNA (B) in PLPs released from EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were collected on day 4 post-exposure from PMA-treated MEG-01 VP30 cells exposed to EBOVΔVP30-GFP at an MOI of 5. EBOV mRNA and gRNA were quantified by RT-qPCR using the indicated specific primer pairs and normalized to the amount in the day 0 samples. Data are presented as means ± SD of three independent experiments performed in triplicate.

Article Snippet: Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics.

Techniques: Expressing, Quantitative RT-PCR

Journal: PLOS Pathogens

Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles

doi: 10.1371/journal.ppat.1013985

Figure Lengend Snippet: A,B. Expression levels of GP and CD41-mCherry in MEG-01 cells stably expressing CD41-mCherry with or without EBOV GP (A), and in PLPs released from these stable cell lines (B). Protein levels were analyzed by immunoblotting using the indicated antibodies. C. Percent of mCherry-positive cells that internalized PLPs containing CD41-mCherry or CD41-mCherry/GP. Huh7 VP30 cells were co-incubated with the indicated PLPs for 1, 3, or 5 h, followed by quantification of mCherry-positive cells by flow cytometry. Data are presented as means ± SD of three independent experiments. Statistical significance was assessed by use of a two-way ANOVA followed by Turkey’s multiple comparisons test. * p < 0.05, **** p < 0.0001.

Article Snippet: Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics.

Techniques: Expressing, Stable Transfection, Western Blot, Incubation, Flow Cytometry

Journal: PLOS Pathogens

Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles

doi: 10.1371/journal.ppat.1013985

Figure Lengend Snippet: Number of GFP-positive cells following co-incubation with PLPs released from EBOVΔVP30-exposed MEG-01 cells (A). PLPs were collected from PMA-treated MEG-01 WT and VP30 cells exposed to EBOVΔVP30-GFP. Huh7 VP30 cells (2 x 10 5 cells) were co-incubated with the indicated PLPs (2 x 10 6 ) for two days. As a control, cells were cultured in the final wash supernatant (B). GFP-positive cells were quantified by flow cytometry. Data are representative of two independent experiments. C-E. EBOVΔVP30 titers in three different VP30-expressing cell types co-cultured with PLPs released from EBOVΔVP30-exposed MEG-01 VP30 cells. PLPs were prepared as described in (A). Huh7 VP30 cells (2 x 10 5 cells, C), HUVEC VP30 cells (2 x 10 5 cells, D), and PMA-differentiated THP-1 VP30 cells (2 x 10 5 cells, E) were co-cultured with the indicated PLPs (2 x 10 6 ). Virus titers on days 2, 4, and 6 were determined using Vero VP30 cells. Data are presented as means ± SD of three independent experiments. The dotted lines indicate the lower limit of detection (1.3 log10 ffu/ml). Statistical significance was assessed by use of a one-way ANOVA followed by Turkey’s multiple comparisons test. *** p < 0.001, **** p < 0.0001.

Article Snippet: Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics.

Techniques: Incubation, Control, Cell Culture, Flow Cytometry, Expressing, Virus

Journal: Scientific reports

Article Title: Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.

doi: 10.1038/s41598-025-88106-0

Figure Lengend Snippet: Fig. 1. Characterization of Ago2-deleted murine platelets. Whole citrated blood collection from Eif2c2fl/fl/Pf4- Cre (Ago2 KO) or Pf4-Cre mice was subject to complete blood count analysis using a HEMAVET analyzer. (A) Platelet counts. (B) Mean platelet volumes (MPV). (C) Platelet counts by sex, shown as box and whisker distribution plots with max/min. (A, C); no significant difference between groups. (D) MPV by sex. (E) Platelet counts following platelet depletion with anti-Gp1bα antibodies. (F) MPV of platelets from (E). (G) Platelet lifespan monitored by non-depleting X488-Gp1bβ antibodies (X488) as described in Methods. Lines in (A- D) indicate median values. Error bars indicate s.e.m. n.s., no significant difference; other data are n.s. unless indicated otherwise. (A-D), n = 25 each; (E-F), n = 3 each. *, p < 0.05; otherwise, no significant differences between groups.

Article Snippet: MEG-01 cells and AGO2 deletion Wild-type MEG-01 cells (ATCC line CRL-2021) were cultured in RPMI medium (Gibco, Grand Island, NY, USA) with 10% FBS (Corning, Corning, NY, USA), 1% non-essential amino acids (Cytiva, Amersham, United Kingdom), and 1% penicillin/streptomycin (GE HealthCare Life Sciences, Chicago, IL, USA) in a humidified, 5% CO2 atmosphere at 37 C. Cells were cultured in EasYFlasks (Thermo Scientific, Waltham, ME, USA) and treated with 50 ng/mL of phorbol myristate acetate (PMA, Sigma-Aldrich, St. Louis, MO) to induce differentiation and platelet-like particle formation.

Techniques: Whisker Assay

Journal: Scientific reports

Article Title: Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.

doi: 10.1038/s41598-025-88106-0

Figure Lengend Snippet: Fig. 2. Altered megakaryocyte development in Ago2 KO mice. (A) Representative femurs and spleens obtained from Pf4-Cre or Ago2 KO mice, sectioned and stained with hematoxylin and eosin. Bars, 200 μm. (B) Quantification of megakaryocytes from images as in (A). (C) Mean forward scatter (FSC) of femur megakaryocytes identified with Cd42d fluorophore-conjugated antibodies by flow cytometry. (D) Ploidy as a function of FSC in megakaryocytes from (C), identified by propidium iodide staining. (E) Quantification of megakaryocyte area from femoral sections from (A), subject to immunohistochemical staining with Cd42d fluorophore-conjugated antibodies, shown + s.e.m. (F) Percentage of femur megakaryocytes per ploidy class from (D). n = 4; *, p < 0.05; **, p < 0.01; ***, p < 0.001. n.s., no significant differences.

Article Snippet: MEG-01 cells and AGO2 deletion Wild-type MEG-01 cells (ATCC line CRL-2021) were cultured in RPMI medium (Gibco, Grand Island, NY, USA) with 10% FBS (Corning, Corning, NY, USA), 1% non-essential amino acids (Cytiva, Amersham, United Kingdom), and 1% penicillin/streptomycin (GE HealthCare Life Sciences, Chicago, IL, USA) in a humidified, 5% CO2 atmosphere at 37 C. Cells were cultured in EasYFlasks (Thermo Scientific, Waltham, ME, USA) and treated with 50 ng/mL of phorbol myristate acetate (PMA, Sigma-Aldrich, St. Louis, MO) to induce differentiation and platelet-like particle formation.

Techniques: Staining, Flow Cytometry, Immunohistochemical staining

Journal: Scientific reports

Article Title: Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.

doi: 10.1038/s41598-025-88106-0

Figure Lengend Snippet: Fig. 3. Sex-selective increased thromboxane reactivity in Ago2-deleted platelets. Platelets from Pf4-Cre (black lines and circles) or Ago2 KO mice (red lines and circles) were subject to agonist stimulation as indicated in the presence of fluorophore-conjugated antibodies against activated αIIbβ3 integrin (Jon/A) and P-selectin (Cd62p), and measured by flow cytometry. (A) U46619. (B) Thrombin. (C) ADP. (D) Convulxin. (A-D) n = 10 each. (E) Data from (A) separated by sex as indicated. n = 5 each. *, p < 0.05; **, p < 0.01; unlabeled data points showed no significant differences between groups. MFI, mean fluorescence intensities, shown ± s.e.m.

Article Snippet: MEG-01 cells and AGO2 deletion Wild-type MEG-01 cells (ATCC line CRL-2021) were cultured in RPMI medium (Gibco, Grand Island, NY, USA) with 10% FBS (Corning, Corning, NY, USA), 1% non-essential amino acids (Cytiva, Amersham, United Kingdom), and 1% penicillin/streptomycin (GE HealthCare Life Sciences, Chicago, IL, USA) in a humidified, 5% CO2 atmosphere at 37 C. Cells were cultured in EasYFlasks (Thermo Scientific, Waltham, ME, USA) and treated with 50 ng/mL of phorbol myristate acetate (PMA, Sigma-Aldrich, St. Louis, MO) to induce differentiation and platelet-like particle formation.

Techniques: Flow Cytometry, Fluorescence

Journal: Scientific reports

Article Title: Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.

doi: 10.1038/s41598-025-88106-0

Figure Lengend Snippet: Fig. 4. Normal hemostasis and clot dynamics following vascular injury in platelet-specific Ago2-deleted mice. (A) Time to initial stoppage of bleeding after 1-mm diameter tail tip amputation in Pf4-Cre (black lines and circles) or Ago2 KO mice (red lines and circles). (B) Rebleeds following primary hemostasis. The line and error bars indicate median and interquartile ranges. (C) Time to hemostasis after rebleeds. The experiment was stopped at 1200 s. No significant differences between groups were observed. n = 20. (D) Data (A-C) separated by sex as indicated, shown ± s.e.m. (E-I) Hemostatic clot dynamics in cremaster muscle arterioles after laser injury, monitored by confocal intravital fluorescence microscopy. 21 injuries in 3 Pf4-Cre male mice and 20 injuries in 3 Ago2 KO male mice. (A) Platelet accumulation (Cd41 area). (B) Cd62p exposure. (C) Fibrin deposition. (H) Area under curve (AUC) for (E). (I) Peak in curves from (E). The line and error bars indicate median and interquartile ranges.

Article Snippet: MEG-01 cells and AGO2 deletion Wild-type MEG-01 cells (ATCC line CRL-2021) were cultured in RPMI medium (Gibco, Grand Island, NY, USA) with 10% FBS (Corning, Corning, NY, USA), 1% non-essential amino acids (Cytiva, Amersham, United Kingdom), and 1% penicillin/streptomycin (GE HealthCare Life Sciences, Chicago, IL, USA) in a humidified, 5% CO2 atmosphere at 37 C. Cells were cultured in EasYFlasks (Thermo Scientific, Waltham, ME, USA) and treated with 50 ng/mL of phorbol myristate acetate (PMA, Sigma-Aldrich, St. Louis, MO) to induce differentiation and platelet-like particle formation.

Techniques: Fluorescence, Microscopy

Journal: Scientific reports

Article Title: Argonaute2 modulates megakaryocyte development and sex-specific control of platelet protein expression and reactivity.

doi: 10.1038/s41598-025-88106-0

Figure Lengend Snippet: Fig. 5. Induced Ago1 expression in Ago2-deleted platelets. (A) Segment from Supplemental Table 1, showing Argonaute peptides identified in Pf4-Cre (WT) and Ago2 KO (KO) platelets by LC/MS/MS. Red boxes indicate Ago2-specific peptides or Ago1-specific peptides as indicated. (B) Platelet lysates as indicated were separated by SDS-PAGE and subject to immunoblotting with the indicated antibodies. Original blots are presented in Supplemental Fig. 4. (C) Densitometric quantitation of immunoblot results from (B). n = 3 each.

Article Snippet: MEG-01 cells and AGO2 deletion Wild-type MEG-01 cells (ATCC line CRL-2021) were cultured in RPMI medium (Gibco, Grand Island, NY, USA) with 10% FBS (Corning, Corning, NY, USA), 1% non-essential amino acids (Cytiva, Amersham, United Kingdom), and 1% penicillin/streptomycin (GE HealthCare Life Sciences, Chicago, IL, USA) in a humidified, 5% CO2 atmosphere at 37 C. Cells were cultured in EasYFlasks (Thermo Scientific, Waltham, ME, USA) and treated with 50 ng/mL of phorbol myristate acetate (PMA, Sigma-Aldrich, St. Louis, MO) to induce differentiation and platelet-like particle formation.

Techniques: Expressing, Liquid Chromatography with Mass Spectroscopy, SDS Page, Western Blot, Quantitation Assay